產(chǎn)品名稱 編號(hào) 產(chǎn)品說明 單位 單價(jià) 加入購物車
GST融合蛋白純化磁珠 70601-5 5 mL, 10%(v/v), 30-150 μm 瓶 ￥580.00
GST融合蛋白純化試劑盒 70601-K10 5 mL, 10%(v/v), 30-150 μm 盒 ￥580.00
GST融合蛋白純化磁珠 70601-100 2×50 mL, 10%(v/v) ,30-150 μm 盒 ￥5000.00
GST融合蛋白純化磁珠 70601-1000 4×250 mL, 10%(v/v), 30-150 μm 盒 ￥30000.00

海貍GST融合蛋白純化磁珠是專為高效、快速純化谷胱甘肽S-轉(zhuǎn)移酶(GST)融合蛋白而設(shè)計(jì)的一種新型功能化材料，可通過磁性分離方式直接從生物樣品中一步純化出高純度的目標(biāo)蛋白，極大地簡(jiǎn)化純化工藝和提高純化效率，適合科研和工業(yè)領(lǐng)域便捷地進(jìn)行GST融合蛋白的純化。
與傳統(tǒng)的柱層析純化方式相比，采用海貍磁珠純化GST融合蛋白，無需對(duì)粗蛋白樣品進(jìn)行多次長(zhǎng)時(shí)間的高速離心，也不需要過濾操作；無需控制流速，更不需要昂貴的層析設(shè)備。樣品與磁珠的特異性結(jié)合、洗滌和目標(biāo)蛋白洗脫變得非常簡(jiǎn)單、快速、易操作，不存在樣品流速控制的瓶頸，對(duì)于熟練的操作者來說，在一個(gè)小時(shí)內(nèi)就能獲得高純度 的目的蛋白，不僅為研究者簡(jiǎn)化了流程，節(jié)省了時(shí)間，降低了成本，并可提高目標(biāo)蛋白的純化效率。使用磁珠能輕 松實(shí)現(xiàn)多樣品平行處理，實(shí)現(xiàn)高通量，也可任意放大樣品純化規(guī)模。


產(chǎn)品名稱BeaverBeads? GSH
磁珠粒徑30μm～150μm
GST融合蛋白結(jié)合量可達(dá)10mg/mL磁珠（100%）
懸液濃度10%(v/v)磁珠懸液
保存液20%(v/v)乙醇
保存溫度4℃～30℃ (長(zhǎng)期保存，建議置于4℃～8℃)
化學(xué)穩(wěn)定性常溫可耐受70%乙醇、6M鹽酸胍、0.1M氫氧化鈉、0.1M醋酸1h


應(yīng)用實(shí)例

1. 磁珠與傳統(tǒng)層析柱純化GST融合蛋白對(duì)比

注：圖中2%、5%、10%、20%和100%指的是分別用谷胱甘肽含量為0.2mM、0.5mM、1mM和10mM的緩沖液對(duì)吸附目標(biāo)蛋白后的磁珠進(jìn)行洗脫。

2.平行操作穩(wěn)定性高，可方便實(shí)現(xiàn)高通量和大規(guī)模蛋白純化

結(jié)果：CV(目標(biāo)蛋白純度） = 1.6%，CV（ 目標(biāo)蛋白載量）= 4.5%。

此外，放大規(guī)模純化樣品時(shí)，只需同比例增加磁珠用量即可，并不影響目標(biāo)蛋白的產(chǎn)率和純度，完全不存在流速和樣品體積的限制，海貍磁珠更加適用于大規(guī)模蛋白純化生產(chǎn)。

3.不同批次間產(chǎn)品使用性能穩(wěn)定，批次間差異小

結(jié)果：CV(目標(biāo)蛋白純度）= 0.7%，CV（ 目標(biāo)蛋白載量）= 4.7%

4.可重復(fù)使用

結(jié)果：同一樣品，重復(fù)使用5次后，其目標(biāo)蛋白載量沒有明顯下降，目標(biāo)蛋白純度基本不變。為了保障純化的質(zhì)量，建議3~5次后，進(jìn)行清洗使用處理。

產(chǎn)品特性

1、蛋白純化快捷

     操作簡(jiǎn)單，一步純化即可得到高純度蛋白；

     方便快捷，一個(gè)小時(shí)輕松實(shí)現(xiàn)蛋白純化。

2、蛋白純化靈活

     可同時(shí)處理多個(gè)樣品，實(shí)現(xiàn)高通量；

     可輕松實(shí)現(xiàn)蛋白濃度和體積的控制，方便后續(xù)樣品精制；

     可輕松實(shí)現(xiàn)對(duì)純化規(guī)模的控制；

     對(duì)微量和低豐度樣品具有良好的純化效果。

3、蛋白純化經(jīng)濟(jì)

     純化流程短，蛋白產(chǎn)量及活性高；

     設(shè)備簡(jiǎn)單，減少了設(shè)備購買及維護(hù)成本，蛋白純化門檻大大降低；

     純化時(shí)間短，節(jié)約了時(shí)間成本；

     可同時(shí)進(jìn)行多樣品處理，實(shí)現(xiàn)自動(dòng)化操作，節(jié)約人工成本；

     能夠重復(fù)使用。

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