| 產(chǎn)品名稱 | 編號(hào) | 產(chǎn)品說(shuō)明 | 單位 | 單價(jià) | 加入購(gòu)物車(chē) |
| His-tag蛋白純化磁珠（IDA-Ni) | 70501-5 | 5 mL, 10%(v/v), 30-150 μm | 瓶 | ￥580.00 | |
| His-tag蛋白純化磁珠（IDA-Ni) | 70501-100 | 2×50 mL, 10%(v/v), 30-150 μm | 盒 | ￥5000.00 | |
| His-tag蛋白純化磁珠（IDA-Ni) | 70501-1000 | 4×250mL, 10%(v/v), 30-150 μm | 盒 | ￥30000.00 | |
| His-tag蛋白純化試劑盒（IDA-Ni) | 70501-K10 | 10次反應(yīng)試劑盒(5 mL) | 盒 | ￥580.00 | |
| His-tag蛋白純化磁珠（IDA-Co) | 70502-5 | 5 mL, 10%(v/v), 30-150 μm | 瓶 | ￥580.00 | |
| His-tag蛋白純化磁珠（IDA-Co) | 70502-100 | 2x50 mL, 10%(v/v), 30-150 μm | 盒 | ￥5000.00 | |
| His-tag蛋白純化磁珠（IDA-Co) | 70502-1000 | 4x250 mL, 10%(v/v), 30-150 μm | 盒 | ￥30000.00 | |
| His-tag蛋白純化試劑盒（IDA-Co) | 70502-K10 | 10次反應(yīng)試劑盒(5 mL) | 盒 | ￥580.00 | |
| His-tag蛋白純化磁珠（NTA-Ni) | 70521P-5 | 5 mL, 25% (v/v), 10-30 μm | 瓶 | 詢價(jià) | |
| His-tag蛋白純化磁珠（NTA-Ni) | 70521P-10 | 10 mL, 25%(v/v), 10-30 μm | 瓶 | 詢價(jià) | |
| His-tag蛋白純化磁珠（NTA-Ni) | 70521P-100 | 2×50 mL, 25%(v/v), 10-30 μm | 瓶 | 詢價(jià) |
BeaverBeads? His-tag Protein Purification磁珠（組氨酸標(biāo)簽蛋白純化瓊脂糖磁珠）是專為高效、快速純化組氨酸標(biāo)簽蛋白而設(shè)計(jì)的一種新型功能化材料，可通過(guò)磁性分離方式直接從生物樣品中一步純化出高純度的目標(biāo)蛋白，顯著地簡(jiǎn)化了純化工藝，降低了設(shè)備需求，適合科研和工業(yè)領(lǐng)域便捷快速地進(jìn)行組氨酸標(biāo)簽蛋白的純化工作。
對(duì)比傳統(tǒng)的過(guò)柱層析純化方式， His-tag Protein Purification磁珠通過(guò)磁性分離方式純化組氨酸標(biāo)簽蛋白，無(wú)需對(duì)粗蛋白樣品進(jìn)行多次費(fèi)時(shí)費(fèi)力的離心、過(guò)濾步驟，無(wú)需裝柱和過(guò)柱層析，無(wú)需昂貴的柱層析設(shè)備，在1小時(shí)內(nèi)便能便捷地獲得高產(chǎn)量和高純度的目的蛋白，且能輕松實(shí)現(xiàn)多樣品平行處理，顯著提高了工作效率，大大降低了設(shè)備、時(shí)間和人工成本.
本產(chǎn)品系列包括鎳離子（Nickel）和鈷離子（Cobalt）兩種金屬離子螯合磁珠。它們?cè)谀繕?biāo)蛋白結(jié)合量及所得目標(biāo)蛋白純度上有所差異，一般推薦客戶使用鎳離子螯合磁珠，大多數(shù)情況下能滿足客戶對(duì)蛋白載量和純度的要求；對(duì)純度要求更高的客戶推薦使用鈷離子螯合磁珠。
His-tag Protein Purification磁珠廣泛適用于細(xì)菌、酵母、昆蟲(chóng)和哺乳動(dòng)物細(xì)胞等分泌或胞內(nèi)表達(dá)的可溶性組氨酸標(biāo)簽蛋白以及變性蛋白的純化。
產(chǎn)品特點(diǎn)
1.可從粗樣品直接純化目標(biāo)蛋白，極大的縮短純化時(shí)間
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2. 可輕松實(shí)現(xiàn)對(duì)目標(biāo)蛋白濃度和體積的控制
通過(guò)調(diào)節(jié)洗脫緩沖液體積的方式，實(shí)現(xiàn)對(duì)目標(biāo)蛋白濃度和體積的控制，無(wú)蛋白損失，且不存在蛋白變性、沉淀的風(fēng)險(xiǎn)。
3. 一步純化即可獲得高純度目標(biāo)蛋白
| 海貍磁珠與市場(chǎng)上進(jìn)口產(chǎn)品相比，一步純化即得到同水平高純度的目標(biāo)蛋白。一個(gè)完整的操作流程即可獲得純度可以達(dá)到95％以上的目標(biāo)蛋白。 |
4.平行操作穩(wěn)定性高，可方便實(shí)現(xiàn)高通量和大規(guī)模蛋白純化
目的蛋白純度和產(chǎn)量的標(biāo)準(zhǔn)差均<5%。
完全不存在流速和樣品體積的限制，適用于高通量和大規(guī)模蛋白純化。
5.可以獲得較高的目標(biāo)蛋白產(chǎn)率
利用海貍磁珠對(duì)粗樣品進(jìn)行純化，一步完整的純化流程之后，90％以上純度的目標(biāo)蛋白，其產(chǎn)率一般達(dá)到70～80％。
6.可重復(fù)使用，再生工藝簡(jiǎn)單
由圖A、B可知，海貍磁珠反復(fù)進(jìn)行多達(dá)六次再生處理，仍能保持較好的目標(biāo)蛋白結(jié)合能力和純度。
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圖A. 再生6次后磁珠結(jié)合目標(biāo)蛋白檢測(cè)圖譜 圖B. 再生6次后磁珠的蛋白結(jié)合量數(shù)據(jù)圖
7. 鎳螯合磁珠與鈷螯合磁珠對(duì)不同分子量的His-tag蛋白純化對(duì)比
? Co離子螯合磁珠蛋白結(jié)合量比Ni離子螯合磁珠稍低，但純度更高；
? Co離子螯合磁珠洗滌和洗脫所用咪唑濃度都比Ni離子螯合磁珠低。
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圖A.鎳和鈷螯合磁珠純化His-tag Lif 蛋白 圖B.鎳和鈷螯合磁珠純化M-MLv蛋白
（分子量～ 50KD）對(duì)比 （分子量～ 70KD）對(duì)比
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